RP-HPLC method was developed for simultaneous estimation of Bacopaside II and Mangiferin using Kromasil RP-18 column with mobile phase used as a combination of Acetonitrile: 10 mM KH2PO4 (40:60 v/v) adjusted to pH 3.0±0.05 with Orthophosphoric acid at 210 nm. RP-HPLC method was developed and validated. The method successfully separated Bacopaside II and mangiferin. The method was found to be linear in range at 10-60 μg/ml of MGN and 20-120 μg/ml of BCP, LOD and LOQ were found to be 0.12 μg/ml and 0.39 μg/ml for MGN and 0.19 μg/ml and 0.57 μg/ml for BCP and the % recovery was found in the range of 98.08-99.18% and 98.05-99.08% and % Assay was found to be 99.25 and 98.40 for MGN and BCP, respectively. The proposed RP-HPLC method was found to be simple, accurate, economical, robust and reproducible.
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